產品介紹
NcmECL Ultra是新賽美生物科技有限公司研發的超敏ECL（Enhanced Chemiluminescent） 化學發光試劑，用于檢測 HRP（辣根過氧化物酶）標記的目的條帶，檢測靈敏度最高可達飛 克（fg）級。NcmECL Ultra 優化了底物的成分，應用了新型的 Enhancer 和氧化劑，發光強度 更高，穩定性更好，不含有毒試劑，安全性更高。
產品特色
? 高靈敏度：最高可達飛克級別，10-15 g ? 安全性高：試劑不含對人體健康有毒的化學物質 ? 信號持續時間長：發光時間長達 30 分鐘以上 ? 穩定性高：室溫運輸穩定性好 ? 經濟節約：較同類進口試劑花費更少，所需抗體少，抗體稀釋度高
操作步驟
1. 準備工作稀釋液，將 NcmECL Ultra Luminol/Enhancer Reagent (A) 和 NcmECL Ultra Stabilized Peroxide Reagent (B) 按體積比例 1:1 混合 注：為了獲得最佳效果，使用前準備稀釋液并立即使用。吸取 NcmECL Ultra 不同 組份時要換移液管，以免污染試劑。 2. 從 TBST 或 PBST 緩沖液的托盤中取出膜，小心移去膜上的多余緩沖液，但不要讓膜干燥。 3. 將膜上有蛋白的一面朝上平整地鋪在一張紙板上，加上配好的工作稀釋液。用量以覆 蓋膜為基準，1ml 工作液可以覆蓋大約 10cm2的膜。 4. 將膜與工作稀釋液孵育 1-5min，以確保整個表面覆蓋。 5. 通過放射自顯影膠片或成像設備獲取信號。對于一個未知的信號，可調節不同曝光的 時間來獲得最佳結果。
保存條件
室溫運輸，請于 4℃保存，有效期為 2 年。
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